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Androgen‐independent basal cell re‐epithelialization, c‐erbB‐2 mRNA expression and androgen‐dependent EGFr mRNA expression in benign prostatic hyperplasia explant cultures treated with finasteride
Author(s) -
Schwartz Simó,
Caceres Carme,
De Torres Inés,
Morote Joan,
RodriguezVallejo José M.,
Gonzalez Jorge,
Reventos Jaume
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980518)76:4<519::aid-ijc13>3.0.co;2-2
Subject(s) - finasteride , dihydrotestosterone , androgen , androgen receptor , endocrinology , medicine , testosterone (patch) , biology , epidermal growth factor , hyperplasia , cancer research , receptor , prostate , cancer , prostate cancer , hormone
We have analyzed the effects of the 5α‐reductase inhibitor, finasteride (MK906), on the mRNA expression of the epidermal growth factor receptor and c‐ erb B‐2 genes, in benign prostatic hyperplasia explant cultures treated with testosterone and with testosterone plus finasteride. A decrease of the epithelial cell content and an androgen‐independent basal cell re‐epithelialization was observed during the first 10 days of culture, suggesting a role of basal cells as stem cells involved in androgen‐independent epithelial regeneration. Using a semi‐quantitative reverse transcription polymerase chain reaction technique, we observed a significant decrease in expression of the epidermal growth factor receptor in the cultures treated with finasteride whereas no effect of finasteride on c‐ erb B‐2 transcription was detected, although the expression of both genes was increased by dihydrotestosterone. Int. J. Cancer 76:519–522, 1998.© 1998 Wiley‐Liss, Inc.