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Expression and tissue localization of matrix metalloproteinase 7 (matrilysin) in human gastric carcinomas. Implications for vessel invasion and metastasis
Author(s) -
Yamashita Kaname,
Azumano Isao,
Mai Masayoshi,
Okada Yasunori
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980417)79:2<187::aid-ijc15>3.0.co;2-7
Subject(s) - matrilysin , pathology , carcinoma , metastasis , immunohistochemistry , matrix metalloproteinase , cancer , lymphovascular invasion , biology , medicine
We examined the production and tissue localization of matrix metalloproteinase‐7 (MMP‐7 = matrilysin) in human gastric carcinomas and analyzed the data in connection with the clinicopathological factors. Sandwich‐enzyme immunoassay for the zymogen of MMP‐7 (proMMP‐7) showed enhanced production of MMP‐7 in carcinoma tissues compared with control normal gastric mucosa. Immunohistochemical studies demonstrated that MMP‐7 is localized predominantly to the carcinoma cells in 71% of the carcinoma samples (30/42 cases). The percentage of immunoreactive carcinoma cells to total carcinoma cells (positive ratio) was significantly higher in intestinal‐type carcinomas (26%, median) than in diffuse‐type carcinomas (3%, median) ( p < 0.05). The positive ratio was markedly higher in carcinoma groups with vascular invasion (28%) or lymphatic permeation (12%) than in those without invasion (6%) or permeation (0%) ( p < 0.05). It was also significantly higher in carcinoma groups with liver (49%) or lymph‐node metastases (15%) than in those without metastases (6 and 2% respectively) ( p < 0.05). Both proMMP‐7 of 28 kDa and active MMP‐7 of 19 kDa were detected in the carcinoma tissues by immunoblotting. Reverse‐transcription‐PCR showed specific amplification in 50% of the carcinoma cases (6/12 cases) and 8% of the normal control specimens (1/12 cases). In situ hybridization demonstrated that the carcinoma cells almost selectively express MMP‐7 mRNA. These data suggest that enhanced production of proMMP‐7 and its activation are implicated in invasion and metastasis of human gastric carcinomas. Int. J. Cancer (Pred. Oncol.) 79:187–194, 1998.© 1998 Wiley‐Liss, Inc.