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Histo‐blood group A/B antigen deletion/ reduction vs . continuous expression in human tumor cells as correlated with their malignancy
Author(s) -
Ichikawa Daisuke,
Handa Kazuko,
Hakomori Senitiroh
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980413)76:2<284::aid-ijc17>3.0.co;2-c
Subject(s) - biology , transfection , integrin , motility , receptor , antigen , cell culture , microbiology and biotechnology , cancer research , immunology , genetics
Deletion or reduction of histo‐blood group A or B antigen in tumors of A or B individuals is clearly correlated with the degree of malignancy and metastatic potential in many types of human cancer. Haptotactic motility of A + H − or B + H − colonic or gastric tumor cell lines produced by transfection of A or B gene was significantly lower than that of parental A − H + or B − H + cells. This is ascribable to reduced function of α3 or α6/β1 integrin receptor as we have recently shown. However, phenotypic changes resulting from gene transfection may not reflect physiological states associated with deletion or reduction vs . continuous expression of A or B antigen in tumors. We now describe the separation and phenotype characterization of A − cells from A + tumor cell lines derived originally from colonic tumors of patients with histo‐blood group A. A + and A − populations were detected in originally A + tumor cell lines SW480 and HT29. A − separated from A + populations isolated from SW480 and HT29 were characterized by greatly enhanced haptotactic motility associated with reduced or deleted A expression at α3, α6, and β1 integrin receptors which control cell motility. Nevertheless, expression of integrin receptors at the surface of A − populations is the same as that for A + populations for both SW480 and HT29 cells. Thus, A vs . H glycosylation in integrin receptors may alter their haptotactic function. Cell proliferation as reflected by 3 H‐thymidine incorporation was also reduced significantly in A + as compared to A − populations. Our findings indicate that the degree of haptotactic motility and proliferation of colonic tumor cells are physiologically associated with the deletion or reduction vs . continuous expression of the histo‐blood group A antigen. Int. J. Cancer 76:284–289, 1998.© 1998 Wiley‐Liss, Inc.