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Gastric M1 mucin, an early oncofetal marker of colon carcinogenesis, is encoded by the MUC5AC gene
Author(s) -
Bara Jacques,
Chastre Eric,
Mahiou Jerôme,
Singh Rupachandra L.,
ForgueLafitte Marie E.,
Hollande Etienne,
Godeau François
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980302)75:5<767::aid-ijc17>3.0.co;2-3
Subject(s) - epitope , microbiology and biotechnology , mucin , antibody , monoclonal antibody , oncofetal antigen , recombinant dna , gene product , biology , immunohistochemistry , gene , muc1 , gene expression , biochemistry , immunology , tumor associated antigen
Gastric M1 mucin and the MUC5AC gene show a similar oncofetal expression in the colon. Our aim was to determine whether M1 mucin is the product of the MUC5AC gene. A recombinant baculovirus encoding the C‐terminal portion of the MUC5AC gene as a fusion protein was isolated and the immunoreactivity of the recombinant mucin (rM) toward M1 antibodies studied. Chicken antibodies also were raised against purified rM. Besides its reactivity with L56/C, a serum recognizing the bacterially expressed MUC5AC gene product, rM was endowed with M1 immunoreactivity: ( i ) rM‐expressing cells were stained specifically with anti‐M1 serum and with the monoclonal antibody (MAb) 21M1, defining the M1‐f epitope; ( ii ) both L56/C and anti‐M1 antibodies recognized the same bands in immunoblots of rM‐containing cell extracts; ( iii ) the 21M1 antibody reacted with rM in an immunoradiometric assay. Among the 7 M1 epitopes, M1‐f was the only one encoded by the 3′ portion of the MUC5AC gene. It was the only epitope detected in a native mucin M1‐derived 170 kDa bromelain proteolytic fragment. Furthermore, the staining patterns of human tissues obtained with either anti‐rM chicken antibodies or anti‐M1 antibodies were identical. We conclude that M1 immunoreactivity is encoded at least in part by the MUC5AC gene. Int. J. Cancer 75:767–773, 1998.© 1998 Wiley‐Liss, Inc.