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Localization of a tumour‐suppressor gene associated with human oral cancer on 7q31.1
Author(s) -
Wang XiaoLi,
Uzawa Katsuhiro,
Miyakawa Akihisa,
Shiiba Masashi,
Watanabe Toshihide,
Sato Takeyuki,
Miya Tsuneo,
Yokoe Hidetaka,
Tanzawa Hideki
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980302)75:5<671::aid-ijc2>3.0.co;2-w
Subject(s) - loss of heterozygosity , biology , carcinogenesis , microsatellite , tumor suppressor gene , epidermoid carcinoma , cancer , chromosome , suppressor , gene , cancer research , microsatellite instability , genetics , allele
To search for the existence of a tumour‐suppressor gene (TSG) associated with oral squamous cell carcinoma (SCC), PCR analysis of microsatellite polymorphisms corresponding to 14 loci which map to chromosome 7q21.3‐qter was performed to screen 35 patients with oral SCC for loss of heterozygosity (LOH). LOH was observed in at least one of the loci in 19 of 34 (55.9%) informative cases. Among the loci tested, frequent LOH was restricted at D7S522 on chromosome 7q31.1, which was measured within 1 cM. Furthermore, we detected microsatellite instability (MI) in 11 of 35 (31.4%) cases tested. Our observations indicate that alterations of chromosome 7q are associated with oral SCC tumorigenesis and that 7q31.1 might harbour at least one putative TSG. Int. J. Cancer 75:671–674, 1998.© 1998 Wiley‐Liss, Inc.