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Transcript levels of aquaporin 1 and carbonic anhydrase IV as predictive indicators for prognosis of renal cell carcinoma patients after nephrectomy
Author(s) -
Takenawa Jun,
Kaneko Yoshiyuki,
Kishishita Masamichi,
Higashitsuji Hiroaki,
Nishiyama Hiroyuki,
Terachi Toshiro,
Arai Yoichi,
Yoshida Osamu,
Fukumoto Manabu,
Fujita Jun
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980220)79:1<1::aid-ijc1>3.0.co;2-5
Subject(s) - renal cell carcinoma , nephrectomy , stage (stratigraphy) , clear cell , clear cell renal cell carcinoma , biology , medicine , aquaporin 1 , carcinoma , kidney cancer , pathology , kidney , carbonic anhydrase , cancer research , oncology , enzyme , water channel , paleontology , biochemistry , mechanical engineering , engineering , inlet
Since failure of differentiation has been suggested to be involved in the neoplastic process and progression of tumors, we evaluated whether the transcript levels of differentiation markers of proximal renal tubular cells, from which renal cell carcinoma (RCC) arises, could be used as prognostic markers. We used Northern blot analysis to study the expression of aquaporin 1 ( aqp1 ) and carbonic anhydrase IV ( ca4 ) genes in 66 paired samples of primary RCC and non‐tumorous kidney tissues. Poor differentiation of tumor cells and non‐clear cell‐subtype RCC were significantly associated with low levels of aqp1 transcripts. When patients were divided into 2 groups according to level of aqp1 transcript in RCC, a low level of aqp1 was significantly associated with unfavorable outcome. Among 18 patients with metastatic RCC and 40 patients with moderately differentiated RCC, those with RCC expressing low levels of aqp1 mRNA demonstrated poorer survival than those with RCC expressing relatively high levels of aqp1. Similarly, decreased expression of ca4 mRNA in RCC was associated with poor survival. On multivariate analysis, transcript levels of aqp1 and stage of the tumor were the independent factors predicting disease‐specific survival. Transcript levels of aqp1 may serve as a new molecular prognostic marker in patients with RCC following nephrectomy. Int. J. Cancer ( Pred. Oncol. ) 79:1–7, 1998.© 1998 Wiley‐Liss, Inc.