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Estradiol and fibulin‐1 inhibit motility of human ovarian‐ and breast‐cancer cells induced by fibronectin
Author(s) -
Hayashido Yasutaka,
Lucas Annick,
Rougeot Christian,
Godyna Svetlana,
Argraves W. Scott,
Rochefort Henri
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980209)75:4<654::aid-ijc26>3.0.co;2-7
Subject(s) - fibulin , fibronectin , motility , extracellular matrix , ovarian cancer , cell culture , cell , estrogen receptor , cancer cell , laminin , biology , cancer research , cell growth , cancer , endocrinology , medicine , chemistry , microbiology and biotechnology , breast cancer , biochemistry , genetics
Ovarian‐cancer cells are characterized by their ability to invade freely the peritoneal cavity. Estradiol stimulates the proliferation of estrogen‐receptor(ER)‐positive ovarian‐cancer cells, as well as expression of fibulin‐1, a fibronectin‐binding extracellular matrix protein. Using a modified Boyden‐chamber assay, we have evaluated the respective roles of estradiol and fibulin‐1 on cell motility, one of the earlier steps of tumor invasion. The effect of estradiol was examined on the random and directional migration of different ER‐positive ovarian‐cancer cell lines. The effect of fibulin‐1 was studied on the motility of the MDA‐MB231 breast‐cancer cell line, which does not express fibulin‐1. We found that when fibronectin (FN) was used as an attractant, estradiol decreased the cell motility of 2 ER‐positive ovarian‐cancer cell lines, BG‐1 and SKOV3, but had no effect on 2 ER‐negative cell lines, PEO14 and MDA‐MB231. The inhibitory effect of estradiol was not observed when collagen (type 1 or 4) or laminin were used as attractants. Fibulin‐1 was found to inhibit haptotactic migration of MDA‐MB231 cells to FN in a dose‐dependent manner. We conclude that both estradiol and fibulin‐1 inhibit cancer cell motility in vitro and therefore have the potential to inhibit tumor invasion. Int. J. Cancer 75:654–658, 1998. © 1998 Wiley‐Liss, Inc.