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Arachidonic‐acid‐selective cytosolic phospholipase A2 is involved in gastrin‐induced AR4‐2J‐cell proliferation
Author(s) -
Ghrib Farida,
Pyronnet Stéphane,
Bastié MarieJosé,
FagotRevurat Pierrette,
Pradayrol Lucien,
Vaysse Nicole
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980119)75:2<239::aid-ijc12>3.0.co;2-8
Subject(s) - arachidonic acid , gastrin , phospholipase a2 , cytosol , endogeny , receptor , phospholipase , endocrinology , cell growth , medicine , biology , chemistry , biochemistry , enzyme , secretion
Gastrin/CCK B G protein‐coupled receptors have been shown to mediate proliferation stimulated by their endogenous ligands. The present study demonstrates the proliferative effect of arachidonic acid on AR4‐2J cells. Gastrin induces an [ 3 H]arachidonic‐acid release in a dose‐dependent manner. The use of a specific inhibitor of cPLA2, AACOCF3 established the involvement of a cPLA2 in the proliferative effect of gastrin. The results also demonstrate that a cytosolic high‐molecular‐weight PLA2 is activated by gastrin in AR4‐2J cells. Int. J. Cancer 75:239–245, 1998. © 1998 Wiley‐Liss, Inc.