Premium
Detailed deletion mapping at chromosome 9p21 in non‐small cell lung cancer by microsatellite analysis and fluorescence in situ hybridization
Author(s) -
Okami Kenji,
Cairns Paul,
Westra William H.,
Linn Jurgen F.,
Ahrendt Steven A.,
Wu Li,
Sidransky David,
Jen Jin
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19971219)74:6<588::aid-ijc5>3.0.co;2-q
Subject(s) - loss of heterozygosity , fluorescence in situ hybridization , biology , chromosome , comparative genomic hybridization , microsatellite , lung cancer , deletion mapping , microbiology and biotechnology , gene mapping , genetics , cancer research , gene , pathology , allele , medicine
We examined 82 cases of primary non‐small cell lung cancer (NSCLC) for loss of heterozygosity (LOH) at the chromosome 9p21‐24 region using 16 microsatellite markers. A total of 52 tumors (63%) displayed LOH, and 25 of these cases displayed LOH for all markers. Two cases had small hemizygous losses confined to the p16 gene and more distal markers, whereas 3 cases had loss proximal to p16 and extended through marker D9S126. This latter region has recently been described as another minimal region of loss at 9p21 in lung cancer. However, homozygous deletion of the p16 gene was observed in 18 of 85 cases, with only 5 cases having large deletions extended into the D9S126 region. Furthermore, we did not observe homozygous deletion at the 9p21 region that excluded the p16 gene. Fluorescence in situ hybridization (FISH) analysis using genomic probes spanning either the p16 or Hel‐N1 (located at D9S126) gene was performed in 14 tumors. The results from FISH correlated with the chromosomal mapping data, suggesting that the p16 region is the major target of deletion at chromosome 9p21 in primary NSCLC. Int. J. Cancer 74:588–592, 1997.© 1997 Wiley‐Liss, Inc.