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Cyclin‐D1 ‐gene amplification is a more potent prognostic factor than its protein over‐expression in human head‐and‐neck squamous‐cell carcinoma
Author(s) -
Kyomoto Ryoichi,
Kumazawa Hirobumi,
Toda Yoshinobu,
Sakaida Noriko,
Okamura Akiharu,
Iwanaga Michitaka,
Shintaku Masayuki,
Yamashita Toshio,
Hiai Hiroshi,
Fukumoto Manabu
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19971219)74:6<576::aid-ijc3>3.0.co;2-r
Subject(s) - cyclin d1 , head and neck squamous cell carcinoma , carcinogenesis , immunohistochemistry , cancer research , biology , gene duplication , cyclin , gene , pathology , cell cycle , medicine , cancer , head and neck cancer , genetics
To evaluate the prognostic significance of cyclin D1 protein/gene expressions in human head‐and‐neck squamous‐cell carcinoma (HNSCC), we examined amplification of the cyclin‐D1 gene ( CCND1 ) by the differential PCR method and over‐expression of cyclin‐D1 protein by immunohistochemistry in 45 paraffin‐embedded sections from HNSCC. Amplification of CCND1 was found in 10 (22%) cases and over‐expression of cyclin D1 was found in 24 (53%) cases. CCND1 amplification was also found in 3 (25%) of 12 cases of dysplastic lesions adjacent to HNSCC. The overall 5‐year survival of patients with CCND1 amplification or with protein over‐production was significantly lower than that of patients without ( p < 0.0001 and p < 0.05, respectively). However, with multivariate analysis, only amplification of CCND1 retained an independent prognostic value ( p = 0.0018). These suggest that CCND1 amplification occurs at early stages of HNSCC tumorigenesis and is a more useful prognostic factor than over‐expression of cyclin D1 in HNSCC. Int. J. Cancer 74:576–581, 1997.© 1997 Wiley‐Liss, Inc.