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Abundance of BRCA1 transcripts in human cancer and lymphoblastoid cell lines carrying BRCA1 germ‐line alterations
Author(s) -
Ribieras Stéphane,
Magdinier Frédérique,
Leclerc Delphine,
Lenoir Gilbert,
Frappart Lucien,
Dante Robert
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19971127)73:5<715::aid-ijc17>3.0.co;2-4
Subject(s) - cell culture , lymphoblast , biology , microbiology and biotechnology , messenger rna , polymerase chain reaction , allele , germ cell tumors , cell , cancer research , genetics , gene , chemotherapy
A competitive polymerase chain reaction has been developed for quantitation of BRCA1 mRNA. In human cancer cell lines, the amount of BRCA1 mRNA is relatively low, ranging from 6 to 38 copies per cell. The decay rate of these transcripts in actinomycin‐treated cells indicates that the half‐life of these molecules is about 4 hr, suggesting that the low concentration of BRCA1 messages is not due to molecular unstability. In human lymphoblastoid cell lines derived from patients carrying germ‐line alterations of BRCA1, the amount of BRCA1 mRNA per cell is lowered only in cell lines exhibiting alterations leading to specific loss of transcripts from the mutated allele. These data indicate that the amount of BRCA1 available in these cells can be related directly to the number of “active” allele. Int. J. Cancer 73:715–718, 1997. © 1997 Wiley‐Liss, Inc.