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Molecular analysis of occupational cancer: infrequent p53 and ras mutations in renal‐cell cancer in workers exposed to gasoline
Author(s) -
Roth Stina,
Partanen Timo,
Suitiala Tuula,
Anttila Sisko,
Ojajärvi Anneli,
Hemminki Kari,
Vainio Harri,
HusgafvelPursiainen Kirsti
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19971114)73:4<492::aid-ijc6>3.0.co;2-v
Subject(s) - cancer , mutation , gene , cancer research , biology , medicine , genetics , pathology
Occupational exposure to gasoline has been identified in several studies as a risk factor for renal‐cell cancer. Cases of renal‐cell cancer with and without work‐related exposure to gasoline or gasoline and diesel fuel were studied for the presence of mutations in the tumour‐suppressor gene p53 (n = 23 exposed and 30 non‐exposed cases studied) and ras oncogene (n = 30 exposed and 36 non‐exposed cases studied). An average cumulative exposure was estimated at 10 ppm‐years benzene among the exposed. Three p53 mutations were detected by denaturing‐gradient gel electrophoresis (DGGE) among the 23 exposed cases (3/23, 13%). Of the non‐exposed referent cases, 4 had a mutation (4/30, 13%). All but one of the cases with a p53 mutation had smoked. A ras gene (K‐ ras or N‐ ras ) mutation was found in 3 (3/66, 4.5%) cases, all of whom were smoker referents. We conclude that p53 and ras mutations are infrequent in renal‐cell cancer associated with occupational exposure to gasoline. However, the majority of the mutations (6/7 for p53, and 3/3 for ras genes) were seen in smokers. Int. J. Cancer 73:492–496, 1997. © 1997 Wiley‐Liss, Inc.