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Detailed microsatellite analysis of chromosome 3p region in non‐papillary renal cell carcinomas
Author(s) -
Chudek Jerzy,
Wilhelm Monica,
Bugert Peter,
Herbers Jutta,
Kovacs Gyula
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19971009)73:2<225::aid-ijc11>3.0.co;2-e
Subject(s) - loss of heterozygosity , breakpoint , biology , deletion mapping , chromosome , microsatellite , papillary renal cell carcinomas , microbiology and biotechnology , genetics , gene mapping , chromosomal translocation , carcinoma , gene , allele
Multiple hemi‐ and homozygous interstitial deletions at chromosome 3p have been found in different types of cancer, including non‐papillary renal cell carcinoma (RCC). To determine the frequency and size of deletions, we have analyzed paired normal and tumor DNA obtained from short‐term cultures of 104 non‐papillary RCCs with 29 microsatellite markers covering the entire chromosome 3p region. Deletion mapping provided evidence for terminal deletion, with the most distal breakpoint between loci D3S1606 and D3S3666 in 94 cases, whereas constitutional heterozygosity was retained at all loci in 4 RCCs. In 6 cases, interstitial deletions were detected. Deletion mapping detected the smallest overlapping region between loci D3S3666 and D3S1560, which corresponds to an approx. 55 cM genetic distance. Int. J. Cancer 73:225–229, 1997. © 1997 Wiley‐Liss, Inc.