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Microsatellite instability in sporadic‐colon‐cancer patients with and without liver metastases
Author(s) -
Chen WeiShone,
Chen Jeou Yuan,
Liu Jacqueline Ming,
Lin Wen Chang,
King Kuang Liang,
WhangPeng Jacqueline,
Yang Wen Kuang
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970822)74:4<470::aid-ijc20>3.0.co;2-c
Subject(s) - microsatellite instability , colorectal cancer , rectum , medicine , gastroenterology , oncology , incidence (geometry) , cancer , dna mismatch repair , germline mutation , germline , microsatellite , biology , mutation , gene , allele , genetics , physics , optics
Microsatellite instability (MSI) is intrinsic to most colorectal carcinomas (CRC) from patients with hereditary non‐polyposis colorectal cancer (HNPCC), reflecting germline mutations in the mismatch‐repair (MMR) genes. Its occurrence and chronological sequence of development in sporadic CRC appears less well defined. To explore the time sequence in acquisition of MSI, and the role it plays during tumor progression in sporadic CRC, we compared the incidence of MSI in tissue samples from 40 Dukes'‐B and 30 Dukes'‐D CRC patients with liver metastases, at 4 different microsatellite loci, representing sites on the APC, DCC and p53 genes respectively as well as the D2S123 site. Among the 30 patients with hepatic metastases, MSI was found in 9 (30%) of the primary, and 13 (43.3%) of the metastatic tumors. In comparison, among the 40 Dukes'‐B CRC, MSI was found in only 8 cases (20%). CRC with MSI were more frequently located in the right colon, less frequently on the left side, and seldom in the rectum. Tumor ploidy analysis shows that 46.2% of Dukes'‐D primary tumors with MSI are diploid (χ 2 = 4.46, p = 0.035). With a mean follow‐up time of 4.2 years for the Dukes'‐B CRC, there were no recurrences in the 8 patients with MSI, whilst 6 (18.8%) relapses occurred amongst the 32 patients without MSI, average time to recurrence being 15 months. In Dukes'‐D CRC, mean survival time for patients with MSI was 37 months (95% CI, 24 to 51 months), for those without MSI 26 months (95% CI, 18 to 35 months), although this was not statistically significant. Our data suggest that tumor progression may involve increased genetic instability. Int. J. Cancer 74:470–474, 1997. © 1997 Wiley‐Liss, Inc.