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MIBG causes oxidative stress and up‐regulation of anti‐oxidant enzymes in the human neuroblastoma cell line SK‐N‐BE(2C)
Author(s) -
Cornelissen Jan,
Van Kuilenburg André B.P.,
Voûte P.A.,
Van Gennip Albert H.
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970729)72:3<486::aid-ijc17>3.0.co;2-a
Subject(s) - neuroblastoma , oxidative stress , enzyme , cell culture , oxidative phosphorylation , chemistry , biochemistry , cancer research , endocrinology , biology , medicine , genetics
We report the effects of meta ‐iodobenzylguanidine (MIBG), a neuroblastoma‐seeking agent, on cell proliferation and several oxidative stress‐related parameters in the human neuroblastoma cell line SK‐N‐BE(2c). MIBG inhibited the proliferation of this cell line in micromolar concentrations. Measurements of the malondialdehyde (MDA) concentrations (a measure of the extent of lipid peroxidation) of cells treated with MIBG showed that increasing concentrations of MIBG led to an increase in MDA levels of the cells. This effect was most pronounced after one day of cellular exposure to MIBG and disappeared after 3 days. Disappearance of the elevated MDA levels caused by MIBG is probably the result of increased activity of the H 2 O 2 detoxifying enzymes, catalase and glutathion peroxidase (GPx). The catalase‐ and GPx‐enzyme activity of cells exposed to MIBG steadily increased with time, reaching a maximum after 4 days. Oxidative stress caused by MIBG thus at first leads to cellular damage (lipid peroxidation) but over a longer period does not lead to decreased proliferation rate of the cells, most likely because of cellular adaptation to increased oxidative stress by up‐regulation of the H 2 O 2 detoxifying enzymes catalase and GPx. Int. J. Cancer 72:486–490, 1997. © 1997 Wiley‐Liss, Inc.