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Stromal fibroblasts influence oral squamous‐cell carcinoma cell interactions with tenascin‐C
Author(s) -
Ramos Daniel M.,
Chen Bing L.,
Boylen Kevin,
Stern Michael,
Kramer Randall H.,
Sheppard Dean,
Nishimura Stephen L.,
Greenspan Deborah,
Zardi Luciano,
Pytela Robert
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970717)72:2<369::aid-ijc28>3.0.co;2-9
Subject(s) - stromal cell , tenascin c , epidermoid carcinoma , biology , integrin , pathology , microbiology and biotechnology , cell culture , cancer research , cell , immunology , medicine , carcinoma , immunohistochemistry , biochemistry , genetics
In this study we identified tenascin‐C (TN‐C) and one of its integrin receptors, αvβ6, in oral squamous‐cell carcinoma (SCC) specimens. Neither TN‐C nor αvβ6 are expressed in normal oral mucosa. We also studied 2 human oral squamous‐cell carcinoma cell lines: the highly invasive HSC‐3 cells, and the poorly invasive SCC‐25 cells. We determined that adhesion of these cells to TN‐C involves both α2 and αv integrins. Migration on TN‐C by oral SCC cells required fibroblast‐conditioned medium and did not occur in its absence. This migration was blocked by anti‐α2 and anti‐αv antibodies and was partially inhibited by antibodies to hepatocyte growth factor, epidermal growth factor and transforming growth factor‐β1. When seeded on TN‐C, the poorly invasive SCC‐25 cells formed αvβ6‐positive focal contacts; the HSC‐3 cells did not. HSC‐3, SCC‐25 and PTF cells secrete TN‐C into the culture medium, as determined by Western blot. However, when HSC‐3 cells were inoculated into the floor of the mouth of nude mice, only murine TN‐C could be identified in the reactive stroma adjacent to the resulting tumor nests, demonstrating that in vivo, HSC‐3 cells do not secrete TN‐C. Our results demonstrate that αvβ6 and tenascin‐C are neo‐expressed in oral squamous‐cell carcinoma, and that the tumor stromal environment is influential in oral SCC behavior. Int. J. Cancer 72:369–376, 1997. © 1997 Wiley‐Liss, Inc.