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Establishment and characterization of 12 uterine cervical‐carcinoma cell lines: Common sequence variation in the E7 gene of HPV‐16‐positive cell lines
Author(s) -
Ku JaLok,
Kim WooHo,
Park HyunSook,
Kang SoonBeom,
Park JaeGahb
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970717)72:2<313::aid-ijc19>3.0.co;2-g
Subject(s) - cell culture , cervical carcinoma , uterine cervix , biology , gene , carcinoma , gene sequence , pathology , genetics , cervical cancer , medicine , cancer , phylogenetic tree
A total of 12 carcinoma cell lines of the human uterine cervix were established from 5 keratinizing and 5 non‐keratinizing squamous‐cell carcinomas, and 2 small‐cell carcinomas. Of these, 10 lines grew as adherent cells and 2 as floating aggregates. All lines showed (i) similarity in morphology to the primary tumor from which they were derived; (ii) high viability with relatively long doubling times (48–96 hr); (iii) absence of Mycoplasma and other bacteria, apart from one Mycoplasma‐contaminated line; (iv) genetic heterogeneity by DNA‐fingerprinting analysis; (v) absence of p53 mutation from exon 4 through 9; and (vi) the presence of HPV DNA sequence. Among the lines, 7 were infected by HPV‐16, 3 by HPV‐18, 1 by HPV‐31, and 1 by HPV‐33; the 2 cell lines derived from small‐cell carcinomas contained HPV‐18. Interestingly, 6 of the 7 cell lines containing HPV‐16‐type DNA harbored the same alteration of E7 at nucleotide position 647 (amino acid 29, A A T |iO A G T, Asn |iO Ser), whereas the 3 HPV‐18‐positive lines did not; 3 cell lines proved to have intact E1/E2 of HPV, suggesting the presence of episomally replicating HPV DNA as well as the integrated form, whereas the other 9 lines were shown to have integrated HPV. Taken together, these cell lines would be very useful for studying the biology of uterine cervical carcinoma. Int. J. Cancer 72:313–320, 1997. © 1997 Wiley‐Liss, Inc.