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Detection of Epstein‐Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV‐infected rhesus monkeys by in situ hybridization
Author(s) -
Pingel Sabine,
Hannig Horst,
MätzRensing Kerstin,
Kaup FranzJosef,
Hunsmann Gerhard,
Bodemer Walter
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970703)72:1<160::aid-ijc23>3.0.co;2-m
Subject(s) - simian immunodeficiency virus , lymphoma , biology , virology , in situ hybridization , antigen , virus , histopathology , immunology , pathology , medicine , gene , genetics , gene expression
SIV infection of macaques is a well‐established animal model for studying the pathogenesis of HIV infection in humans. During the course of SIV infection, up to 40% of cynomolgus macaques ( Macaca fascicularis ) develop SIV‐associated non‐Hodgkin's lymphomas. In the present study, we characterized malignant lymphomas of SIV mac 251/32H‐infected rhesus macaques ( Macaca mulatta ) of our cohort in terms of clinical outcome, histopathology and EBV association. Histopathologic changes of lymphoid malignancies were classified according to the Kiel classification. For detection of the EBV‐encoded small RNAs EBER1 and EBER2, a method of non‐isotopic in situ hybridization was established. The presence of EBNA‐2 antigens was assessed by immunohistochemistry. Seven of 43 rhesus macaques developed highly malignant B‐cell lymphomas of the centroblastic, immunoblastic and Burkitt subtypes within 18–29 months post‐experimental SIV infection. In situ hybridization revealed the presence of small EBER1 and ‐2 RNAs in 6 of 7 disease cases. EBNA‐2 antigens could be demonstrated in only 4 of 7 tissue specimens. As expected, the Burkitt‐type of lymphoma was negative for EBNA‐2 antigen staining. In accordance with findings on SIV‐associated lymphomas of cynomolgus macaques, infection with an EBV‐related herpesvirus could be demonstrated in almost 90% of lymphomas in SIV‐infected rhesus macaques. In contrast, the presence of EBV in lymphomas had been documented previously in only 30–40% of HIV‐infected patients. Further studies should thus define the precise role of herpesvirus infection for lymphomagenesis in SIV‐ and HIV‐induced immunodeficiency. Int. J. Cancer 72:160–165, 1997. © 1997 Wiley‐Liss Inc.