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Deletion analysis at the DEL ‐27, APC and MTS 1 loci in bladder cancer: LOH at the DEL ‐27 locus on 5p13‐12 is a prognostic marker of tumor progression
Author(s) -
Böhm Malte,
Kirch Heide,
Otto Thomas,
Rübben Herbert,
Wieland Ilse
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970620)74:3<291::aid-ijc10>3.0.co;2-f
Subject(s) - bladder cancer , tumor progression , locus (genetics) , pathology , biology , cancer research , medicine , cancer , oncology , genetics , gene
Inactivation of relevant tumor‐suppressor genes by allelic or homozygous deletion is a characteristic event in tumor cells. Here, the prognostic value of allelic deletions on 5p13‐12 at the putative del ‐27 tumor‐suppressor locus and in the APC tumor‐suppressor gene on 5q21, as well as homozygous deletions of the MTS 1 ( p16 INK4 , CDKN 2) tumor‐suppressor gene on 9p21 was assessed in 87 bladder cancers using microdissection and PCR‐based assays. Tumor‐specific LOH was detected in 10 of 38 (26%, del ‐27), and 15 of 30 (50%, APC ) informative specimens. Homozygous deletion of the MTS 1 gene was detected in 33% of 84 tumors investigated. These deletion frequencies implicate the 3 tumor‐suppressor regions in the genesis of transitional‐cell carcinoma. In contrast to deletions of the APC or MTS 1 genes, LOH at the del ‐27 locus correlated with tumor progression. This suggests that loss of the putative tumor‐suppressor gene DEL ‐27 is involved in an aggressive behavior of the tumor cells and appears to be a prognostic marker for the clinical outcome of patients with transitional‐cell carcinoma. Int. J. Cancer 74:291‐295, 1997. © 1997 Wiley‐Liss, Inc.