Premium
Interleukin‐10 is a growth factor for human melanoma cells and down‐regulates HLA class‐I, HLA class‐II and ICAM‐1 molecules
Author(s) -
Yue Feng Yun,
Dummer Reinhard,
Geertsen Ralf,
Hofbauer Günther,
Laine Elisabeth,
Manolio Silvana,
Burg Günter
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970516)71:4<630::aid-ijc20>3.0.co;2-e
Subject(s) - autocrine signalling , melanoma , human leukocyte antigen , cytokine , biology , cancer research , microbiology and biotechnology , immunology , cell culture , antigen , genetics
IL‐10 is a cytokine which shows various effects including inhibition of T‐cell proliferation or HLA‐dependent antigen presentation. In this study, we analysed the effects of exogenous or autocrine IL‐10 on proliferation and expression of immunocritical surface molecules. Fourteen cultures of human melanoma cells were established from primary melanomas, locoregional lymph‐node or distant metastases. In 5 melanoma cell cultures, proliferation in the presence of IL‐10, anti‐IL‐10 antibodies (Ab) or control Ab was assessed with colorimetric and [ 3 H]thymidine uptake assays. Flow cytometric analysis was used to quantify the expression of human leukocyte antigen (HLA) class‐I, HLA class‐II and intercellular adhesion molecule (ICAM)‐I and the IL‐10 receptor (IL‐10R). IL‐10 production of melanoma cells was documented by RT‐PCR and IL‐10 protein was detected in the supernatants by means of ELISA. IL‐10 enhanced proliferation and prolonged survival of melanoma cells in 5 out of 5 cultures. Anti‐IL‐10 Ab decreased proliferation. IL‐10R expression was found in 12 out of 14 (86%) melanoma cell cultures. The expression of HLA‐I, HLA‐II and ICAM‐I on all melanoma cells that were positive for IL‐10R showed a reduction of 10‐60% by IL‐10, whereas the surface levels of HLA‐I, HLA‐II and ICAM‐I in 5 out of 5 cell cultures revealed an increase of 10‐170% by anti‐IL‐10 Ab. These findings suggest that IL‐10 is an autocrine growth factor with significant impact on immunocritical molecules in melanoma. IL‐10 effects have to be considered when planning therapeutic immunointerventions in melanoma patients. Int. J. Cancer 71:630‐637, 1997. © 1997 Wiley‐Liss, Inc.