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MHC‐dependent cytolysis of autologous tumor cells by lymphocytes infiltrating urothelial carcinomas
Author(s) -
Housseau Franck,
Zeliszewski Dominique,
Roy Maguy,
Paradis Valerie,
Richon Sophie,
Ricour Alice,
Bougaran Joelle,
Prapotnich Dominique,
Vallancien Guy,
Benoit Gérard,
Desportes Laurent,
Bedossa Pierre,
Hercend Thierry,
Bidart JeanMichel,
Bellet Dominique
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970516)71:4<585::aid-ijc13>3.0.co;2-b
Subject(s) - tumor infiltrating lymphocytes , ctl* , cd8 , mhc class i , cytolysis , cytotoxic t cell , biology , cancer research , major histocompatibility complex , immune system , immunology , in vitro , biochemistry
Tumor‐infiltrating lymphocytes (TIL) were grown from 23 urothelial carcinomas. Phenotyping analysis showed that the TIL cultures were mainly CD3 + . Although CD4 + and CD8 + T‐cell sub‐sets were grown in culture, CD4 + T‐cell sub‐sets predominated over CD8 + T cells. Immunohistochemical studies performed on 5 tumor specimens confirmed this observation, and indicated that CD4 + T cells surrounded the tumor islets, whereas CD8 + T lymphocytes were localized among the tumor cells. Five short‐term carcinoma cell lines established from these urothelial tumors were used as target cells in cytolysis assays in order to investigate the functional anti‐tumor activity of autologous TIL. TIL from 4/5 tumors were lytic and 3 TIL lines displayed MHC‐class‐I‐dependent cytotoxicity directed against autologous tumor cells. CD4 + T‐cell‐depletion experiments performed on TIL line 07 confirmed that CD8 + MHC‐class‐I‐dependent CTL were the predominant effectors. Finally, experiments performed on 6 allogeneic urothelial‐cancer cell lines matched for HLA‐class‐I molecules showed that TIL07 exhibited selective lytic activity toward tumor 07. These data indicate that CD8 + MHC‐class‐I‐dependent CTL present in urothelial carcinomas are functional and may participate in the anti‐tumor immune response. Int. J. Cancer 71:585‐594, 1997. © 1997 Wiley‐Liss, Inc.