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Translation initiation factor eIF‐4A1 mRNA is consistently overexpressed in human melanoma cells in vitro
Author(s) -
Eberle Jürgen,
Krasagakis Konstantin,
Orfanos Constantin E.
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970502)71:3<396::aid-ijc16>3.0.co;2-e
Subject(s) - in vitro , messenger rna , translation (biology) , initiation factor , biology , gene , genetics , cancer research , microbiology and biotechnology , medicine
Abstract The oncogenic potential of translation initiation factors (eIF‐4E and eIF‐2α) has been described in previous studies leading to the definition of translational oncogenes. Two previously isolated cDNA clones, expressed differently in human melanoma cells and normal human melanocytes, were identified in this study as coding for the translation initiation factor eIF‐4AI. Northern‐blot analysis revealed consistent overexpression of eIF‐4AI mRNA in a panel of 14 melanoma cell lines (on an average 5.6 times higher than in cultures of normal human melanocytes). In contrast, the mRNAs of the other group‐4 translation initiation factors (eIF‐4A2, eIF‐4B, eIF‐4E and eIF‐4γ) were less and not consistently elevated. Cultures of congenital melanocytic nevi exhibited intermediate expression of eIF‐4AI. Thus, eIF‐4AI overexpression seems to be an important feature of melanoma cells and might contribute to their malignant transformation. Int. J. Cancer 71:396‐401, 1997. © 1997 Wiley‐Liss Inc.