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Glucose‐transporter‐type‐I‐gene amplification correlates with Sialyl‐Lewis‐X synthesis and proliferation in lung cancer
Author(s) -
Ogawa Junichi,
Inoue Hiroshi,
Koide Shirosaku
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970422)74:2<189::aid-ijc9>3.0.co;2-v
Subject(s) - lung cancer , gene duplication , cancer research , glucose transporter , gene , biology , transporter , cell growth , microbiology and biotechnology , chemistry , medicine , endocrinology , genetics , insulin
Increased glucose transport is a common characteristic of most tumors. To examine the role of elevated glucose uptake in lung cancer, we performed PCR amplification of 2 facilitative glucose transporter genes ( GLUT 1 and GLUT 3) and immunohistochemical staining for GLUT1, proliferating cell nuclear antigen (PCNA), and sialyl Lewis × (sLe x ) on tumor specimens from 327 patients with lung cancer who underwent surgical resection from 1980 to 1993. To evaluate the relationship between GLUT, α‐2,3‐sialyltransferase ( ST ), and α‐1,3‐fucosyltransferase ( Fuc‐T ) genes, PCR amplification of the ST3N and Fuc‐TVII also was performed. Amplification of GLUT 1 was significantly greater than that of GLUT 3. GLUT 1 and GLUT 3 amplification correlated with PCNA staining ( p < 0.01). In addition, GLUT 1 amplification correlated with the grading of sLe x staining as well as with the grading of GLUT1 staining ( p < .03, p < 0.01). GLUT 1 was co‐amplified with ST3N and Fuc‐TVII genes, which are involved in the synthesis of sLe x ( p < 0.01). The survival of patients whose tumors showed GLUT 1 amplification was significantly shorter than that of patients whose tumors did not ( p < 0.01). In a multivariate analysis of survival, GLUT 1 remained a statistically significant prognostic factor. Our results suggest that GLUT 1 amplification may participate in sLe x synthesis as well as in proliferation, and may be of prognostic value in lung cancer. Int. J. Cancer 74:189–192, 1997. © 1997 Wiley‐Liss, Inc.