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Metanestin, a glycoprotein with metastasis‐associated expression in transitional cell carcinoma of the urinary bladder
Author(s) -
Takemoto Masakazu,
Shirahama Tsutomu,
Miyauchi Teruo,
Matsusako Tetsusi,
Kaneda Norio,
Muramatsu Hisako,
Ozawa Masayuki,
Ohi Yoshitada,
Muramatsu Takashi
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970220)74:1<7::aid-ijc2>3.0.co;2-6
Subject(s) - metastasis , transitional cell carcinoma , urinary bladder , glycoprotein , urinary system , carcinoma , cancer research , medicine , pathology , biology , bladder cancer , microbiology and biotechnology , cancer
A 60‐kDa glycoprotein named metanestin was identified by molecular cloning. The glycoprotein had a twice‐repeated motif of Pro‐Gly‐Pro‐Gly and carried metastasis‐associated carbohydrate epitopes. The antibody to the protein portion of metanestin strongly reacted with lymph‐node metastasis of transitional‐cell carcinoma of the urinary bladder, but the reactivity to the primary tumor was generally weaker and the normal urothelium was scarcely reactive. Thus both metanestin and the carbohydrate on it showed metastasis‐associated expression. In addition, we identified a 100‐kDa glycoprotein with a 4‐times‐repeated motif of Pro‐Ala‐Pro‐Ala. The antibody to the 100‐kDa glycoprotein showed reactivity similar to that to metanestin. Int. J. Cancer 74:7–14. © 1997 Wiley‐Liss, Inc.