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Establishment and characterization of human gastric carcinoma cell lines
Author(s) -
Park JaeGahb,
Yang HanKwang,
Kim Woo Ho,
Chung JuneKey,
Kang MyungSoo,
Lee JaeHo,
Oh Jae Hwan,
Park HyunSook,
Yeo KyongSook,
Kang Shin Hyuck,
Song SangYong,
Kang Yun Kyung,
Bang YungJue,
Kim Yong Il,
Kim JinPok
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970207)70:4<443::aid-ijc12>3.0.co;2-g
Subject(s) - microbiology and biotechnology , cell culture , biology , cell , mutation , lysis , cancer research , gene , genetics
We report 8 newly established gastric‐carcinoma cell lines (SNU‐216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, αFP, and CA 19‐9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53 and c‐Ki ‐ ras gene mutations were screened and confirmed by sequencing. The cell lines, derived from tumors with moderate differentiation, grew as a diffuse monolayer, and those from tumors with poor differentiation and minimal desmoplasia grew exclusively as non‐adherent. Out of the 8 gastric‐cancer cell lines, 5 had detectable levels of CEA both in supernatant and in cell lysate; there was no expression or secretion of αFP in these cells; 4 cell lines showed high levels of CA 19‐9 in cell pellets. All cell lines except SNU‐484 had high concentrations of TPA both in cell lysate and in supernatants. p53 mutation was found in 6 cell lines (75%): 2 (SNU‐216 and SNU‐668) had mutations in exon 6, and other 3 in exon 8. The c‐Ki ‐ ras mutation was found in 2 cell lines (25%), SNU‐601 and SNU‐668. The former showed GGT‐to‐GAT transition mutation at codon 12, while the latter showed CAA‐to‐AAA transversion mutation at codon 61. DNA profiles using restriction endonuclease Hinfl and polymorphic DNA probes ChdTC‐15 and ChdTC‐114 showed different unique patterns; which suggests that these cell lines are unique and not cross‐contaminated. We believe that the newly characterized gastric‐cancer cell lines presented in this paper will provide a useful in vitro model for studies related to human gastric cancer. Int. J. Cancer, 70:0–0, 1997. © 1997 Wiley‐Liss, Inc.