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Human ovarian‐carcinoma cell lines express IL‐4 and IL‐13 receptors: Comparison between IL‐4‐ and IL‐13‐induced signal transduction
Author(s) -
Murata Takashi,
Obiri Nicholas I.,
Puri Raj K.
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970117)70:2<230::aid-ijc15>3.0.co;2-m
Subject(s) - phosphorylation , signal transduction , tyrosine phosphorylation , cancer research , cell culture , biology , ovarian carcinoma , receptor , janus kinase 1 , ovarian cancer , janus kinase 2 , tyrosine kinase 2 , medicine , microbiology and biotechnology , endocrinology , janus kinase , cancer , biochemistry , growth factor , platelet derived growth factor receptor , genetics
We have reported that human ovarian‐carcinoma cell lines express high‐affinity IL‐4 receptor. Since IL‐4R has been hypothesized to share a chain with IL‐13R, we investigated whether ovarian cancer cells express IL‐13 receptor. In the present study, we report that the ovarian‐carcinoma cell lines IGROV‐1 and PA‐1 express varying numbers of high‐affinity IL‐13 receptors. Furthermore, IL‐13 inhibited the binding of IL‐4 on both ovarian‐carcinoma cell lines, while IL‐4 did not inhibit IL‐13 binding on IGROV‐1 cell line. IL‐13 and IL‐4 induced the phosphorylation of JAK1, JAK2 and Tyk2 Janus kinases in PA‐1 cells. In contrast, JAK3 tyrosine kinase was expressed in PA‐1 cells, but IL‐4 or IL‐13 did not augment its phosphorylation. In IGROV‐1 cells, Tyk2 was constitutively phosphorylated and this phosphorylation was augmented by IL‐4 or IL‐13. JAK1 and JAK2 but not JAK3 were expressed but only JAK2 was faintly phosphorylated in response to either IL‐13 or IL‐4 respectively. IRS (insulin‐receptor substrate)‐1 and IRS‐2 were also phosphorylated constitutively in both ovarian cancer cell lines examined, but only the phosphorylation of IRS‐1 was augmented in response to IL‐4 or IL‐13. STAT6 was phosphorylated and activated in response to IL‐4 and IL‐13 in all cell lines examined. Our results demonstrate that ovarian cancer cell lines may express 2 types of IL‐13R and the IL‐13‐ or IL‐4‐induced signaling patterns may be slightly different in each type of receptor. Int. J. Cancer, 70:230–240, 1997. © 1997 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.