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Dendritic epidermal T‐cell involvement in induction of CD8 + T cell‐mediated immunity against an ultraviolet radiation‐induced skin tumor
Author(s) -
Cavanagh Lois L.,
Barnetson Ross StC.,
Basten Antony,
Halliday Gary M.
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970106)70:1<98::aid-ijc15>3.0.co;2-9
Subject(s) - immune system , cd8 , biology , immunity , antigen , immunology , fibrosarcoma , acquired immune system , dendritic cell , antigen presentation , t cell , cancer research , cytotoxic t cell , in vitro , biochemistry , genetics
Murine epidermis contains 2 distinct cell populations which contribute to the skin immune system, Langerhans cells (LC), and dendritic epidermal T cells (DETC). LCs are important in the induction of immunity against a wide range of antigens; however, the function of DETC is unclear. To investigate the roles of these epidermal cells (EC) in protective antitumor immunity, an in vivo model of an ultraviolet radiation‐induced fibrosarcoma, UV‐13‐1, was used. Mice were immunized with tumor antigen‐pulsed EC followed 10 days later by an injection into the ear of 10 5 tumor cells, which did not lead to formation of a detectable tumor, but was intended to simulate the influence of a developing tumor on the ensuing immune response. The mice were then challenged with 2 × 10 6 viable tumor cells in each flank, sufficient to result in growth of a measurable tumor. Protective immunity was induced by DETC, and shown to be long‐lasting, with tumors inoculated 160 days after immunization being effectively rejected. The effector cells responsible for protective immunity were CD8 + T cells. Delayed‐type hypersensitivity generated by tumor antigen‐pulsed EC was dependent on Lcs, with no involvement of DETCs. This response, in contrast to that of DETC, required prior culture of EC with GM‐CSF, but failed to inhibit tumor growth or incidence. Thus DETC and LC can both activate antitumor immune responses, although only the DETC‐dependent response results in protective immunity in the presence of a developing tumor. © 1997 Wiley‐Liss, Inc.