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Increased analytical sensitivity of RT‐PCR of PSA mRNA decreases diagnostic specificity of detection of prostatic cells in blood
Author(s) -
Henke Wolfgang,
Jung Monika,
Jung Klaus,
Lein Michael,
Schlechte Horst,
Berndt Christoph,
Rudolph Birgit,
Schnorr Dietmar,
Loening Stefan A.
Publication year - 1997
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19970106)70:1<52::aid-ijc8>3.0.co;2-5
Subject(s) - prostate cancer , messenger rna , medicine , prostate , hyperplasia , real time polymerase chain reaction , polymerase chain reaction , reverse transcriptase , reverse transcription polymerase chain reaction , cancer , prostate specific antigen , prostatic disease , pathology , biology , gene , biochemistry
The diagnostic specificity of the detection of disseminated prostatic cells by reverse‐transcriptase polymerase chain reaction (RT‐PCR) of PSA mRNA was investigated. A sensitive nested PCR was developed. In blood samples from 10 healthy female and 10 healthy male persons examined by RT‐PCR, mRNA of PSA was detected 3 times in each group. In the groups of patients suffering from benign prostate hyperplasia and prostate cancer, 6 of 11 and 5 of 12, respectively, gave positive RT‐PCR results. With increasing analytical sensitivity of the RT‐PCR of PSA mRNA, the diagnostic specificity of the assay is decreased. Further development of this diagnostic method requires the introduction of the quantitative PCR which may make possible discrimination between prostatic and non‐prostatic source of PSA mRNA by quantification. © 1997 Wiley‐Liss, Inc.