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The roles of Fas and perforin in LAK T‐cell/bispecific antibody‐mediated killing of the murine B‐lymphoma cells, BCL 1
Author(s) -
Armstrong John M.,
Vitetta Ellen S.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961211)68:6<822::aid-ijc22>3.0.co;2-0
Subject(s) - perforin , lymphokine activated killer cell , immunology , lymphokine , fas ligand , cancer research , biology , t cell , interleukin 21 , immune system , programmed cell death , apoptosis , cd8 , biochemistry
The aim of this study was to construct bispecific F(ab′) 2 [anti‐CD3 × anti‐BCL 1 idiotype (Id)] Abs (BsAbs) which would enable lymphokine‐activated killer (LAK) T cells to kill Id + mouse BCL 1 lymphoma cells, and to determine the mechanism(s) underlying cell death. Using 4‐day activated LAK T cells from either perforin‐knockout mice or FasL‐deficient gld mice, we show that the Fas pathway, but not perforin, is required for BsAb‐mediated LAK T‐cell‐induced killing of BCL 1 cells. © 1996 Wiley‐Liss, Inc.