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Over‐expression of a novel member of the immunoglobulin superfamily in Min mouse intestinal adenomas
Author(s) -
Chadéneau Corinne,
LeCabellec Marité,
LeMoullac Béatrice,
Meflah Khaled,
Denis Marc G.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961211)68:6<817::aid-ijc21>3.0.co;2-w
Subject(s) - biology , complementary dna , familial adenomatous polyposis , microbiology and biotechnology , adenomatous polyposis coli , immunoglobulin superfamily , gene , messenger rna , gene expression , in situ hybridization , glycoprotein , germline , antibody , colorectal cancer , cancer , genetics
Germline mutations of the APC (adenomatous polyposis coli) gene lead to multiple intestinal tumors in familial adenomatous polyposis patients and in Min (multiple intestinal neoplasia) mice. Consequently, these mice provide an excellent model for familial colon cancer. We have identified an M r approx. 66 kDa glycoprotein which is preferentially expressed at the cell surface of cell lines established from chemically induced rat colon carcinomas. Cloning of the corresponding Tage4 cDNA has revealed that this protein contains the conserved amino acids characteristic of members of the immunoglobulin gene superfamily. Here, we analyze expression of the mouse Tage4 gene in Min mouse intestinal adenomas. RT‐PCR analysis allowed us to detect expression of this gene in all the mouse adenomas tested. In contrast, lower levels of Tage4 mRNA were found in the intestinal tract and barely detectable levels in other tissues of normal mice. Furthermore, Tage4 mRNA was detected in a series of mouse intestinal adenomas by in situ hybridization. A strong signal was seen in the samples analyzed. © 1996 Wiley‐Liss, Inc.