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Over‐expression of bcl‐x L gene in human gastric adenomas and carcinomas
Author(s) -
Kondo Shinya,
Shinomura Yasuhisa,
Kanayama Shuji,
Higashimoto Yoshifumi,
Miyagawa JunIchiro,
Minami Takeshi,
Kiyohara Tatsuya,
Zushi Shinichiro,
Kitamura Shinji,
Isozaki Koji,
Matsuzawa Yuji
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961211)68:6<727::aid-ijc6>3.0.co;2-5
Subject(s) - bcl xl , carcinogenesis , pathology , carcinoma , gene expression , gene , stomach , immunohistochemistry , messenger rna , biology , cancer research , medicine , apoptosis , programmed cell death , biochemistry
The present study was designed to clarify whether bel‐xL is involved in the development of carcinoma in the stomach. Levels of bel‐x L and bc1‐2 mRNA were determined by a reverse‐transcription/polymerase‐chain reaction in endoscopic gastric biopsy specimens from 10 control subjects, 11 patients with adenomas and 14 patients with carcinomas. In 6 of 11 adenomas, 5 of 8 early carcinomas and 3 of 6 advanced carcinomas, the bel‐x L gene was over‐expressed. In carcinomas, overexpression of the bel‐x L gene was observed in 6 of 9 intestinaltype carcinomas and 2 of 5 diffuse‐type carcinomas. No correlation was observed between bel‐x L and bc1‐2 gene expression. In cases in which the bel‐x L gene was over‐expressed, an apparent increase in the protein level of Bcl‐x L was observed by immunoblot analysis and intense Bcl‐x immunoreactivity was detected immunohistochemically within the tumor cells. In conclusion, we showed that bel‐x L is over‐expressed in gastric carcinomas at both the RNA and protein levels, suggesting that overexpression of bel‐x L may play a role in gastric carcinogenesis. © 1996 Wiley‐Liss, Inc.