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Effect of transfected interleukin‐6 in non‐tumorigenic and tumorigenic rat urothelial cell lines
Author(s) -
Okamoto Masato,
Oyasu Ryoichi
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961127)68:5<616::aid-ijc11>3.0.co;2-#
Subject(s) - transfection , carcinogenesis , biology , cell culture , cancer research , in vivo , cell growth , cytokine , microbiology and biotechnology , in vitro , cancer , immunology , biochemistry , genetics
It has been suggested that interleukin (IL)‐6 plays a significant role in bladder carcinomas. We conducted the present experiment to determine whether over‐expressed IL‐6 enhanced the tumorigenicity of a weakly tumorigenic rat bladder carcinoma, and whether it was sufficient to induce a tumorigenic phenotype in a non‐tumorigenic, anchorage‐dependent rat urothelial cell line, MYP3. P3M6‐10 and P3M6‐12 (anchorage‐independent but non‐tumorigenic) and MYP3T6 (anchorage‐independent and tumorigenic) were isolated from MYP3 after treatment with MNU in vitro . None of these clones produced IL‐6. The cells were transfected with an expression vector containing human IL‐6 cDNA. The transfectants secreted a large amount of IL‐6. In MYP3T6t over‐expression of IL‐6 enhanced tumorigenicity markedly in nude mice, as evidenced by acceleration of the growth rate in vivo as well as of anchorage‐dependent and ‐independent growth. In P3M6‐10 and P3M6‐12, the introduced IL‐6 cDNA markedly enhanced their growth potential, both on a plastic surface and in soft agar, but did not induce a tumorigenic potential. In MYP3, introduced IL‐6 weakly enhanced their growth on a plastic surface, but caused neither tumorigenesis in nude mice nor colony formation in soft agar. Expression of gpl30 , an IL‐6 signal transducer, was increased in IL‐6‐expressing clones, especially in the P3M6‐10 and MYP3T6. We conclude that acquisition of the ability to synthesize endogenous IL‐6 markedly accelerates the growth rate of weakly tumorigenic rat urothelial cells, but is not suffficient to induce a tumorigenic phenotype in non‐tumorigenic cells. © 1996 Wiley‐Liss, Inc.