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Induction of apoptosis by ribosome‐inactivating proteins and related immunotoxins
Author(s) -
Bolognesi Andrea,
Tazzari Pier Luigi,
Olivieri Fabiola,
Polito Letizia,
Falini Brunangelo,
Stirpe Fiorenzo
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961104)68:3<349::aid-ijc13>3.0.co;2-3
Subject(s) - immunotoxin , ribosome inactivating protein , propidium iodide , microbiology and biotechnology , apoptosis , biology , dna fragmentation , monoclonal antibody , saporin , chemistry , antibody , biochemistry , programmed cell death , ribosome , immunology , rna , gene
Immunotoxins have been prepared with 3 ribosome‐inactivating proteins (RIPs), namely, momordin, pokeweed anti‐viral protein from seeds (PAP‐S) and saporin, linked to the Ber‐H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium‐iodide staining and fluoresceine‐isothiocyanate conjugate dUTP incorporation). The AC 50 (concentration causing apoptosis in 50% of the cells) is in the range 10 ‐8 to 10 ‐7 M in the case of RIPs, and 10 ‐11 to 10 ‐10 M in the case of the immunotoxins. © 1996 Wiley‐Liss, Inc.