Premium
Increased matrix metalloproteinase‐9 secretion in short‐term tissue cultures of prostatic tumor cells
Author(s) -
Festuccia Claudio,
Bologna Mauro,
Vicentini Carlo,
Tacconelli Antonella,
Miano Roberto,
Violini Stefania,
Mackay Andrew R.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961021)69:5<386::aid-ijc6>3.0.co;2-1
Subject(s) - matrix metalloproteinase , secretion , prostate , malignancy , pathology , hyperplasia , biology , carcinoma , immunohistochemistry , medicine , cancer research , endocrinology , cancer
We have examined the expression of 2 tumor‐associated metalloproteinases, MMP‐2 and MMP‐9, in 48 primary cultures of prostatic carcinoma (PRCA) and 33 cultures of benign prostatic hyperplasia (BPH). PRCA cultures secrete significantly more MMP‐9 than their benign counterparts. Secreted MMP‐2 did not differ significantly in cultures but was lower in PRCA cultures. Two cultures of benign origin exhibited high MMP‐9 secretion and growth patterns consistent with a malignancy. Both cases were followed and successively re‐evaluated histologically and rediagnosed as organ‐confined PRCA. MMP expression in culture may be of predictive value in the identification of incidental PRCA. MMP‐9 secretion and its ratio with MMP‐2 were highest in epithelial cultures from invasive, metastatic tumors when compared both to disease confined to prostate gland and to locally extensive disease. MMP‐9 secretion was greatest also in cultures derived from tissues of high Gleason histological grade. Active MMP‐9 species were detected in 15 cultures (31%) of PRCA. Active MMP‐2 species were observed in cultures of both BPH and PRCA origin in almost the same amounts. Although average levels were not significantly different, as a ratio to proform species, a significant elevation was observed in cultures of PRCA origin. We propose, therefore, that an elevated expression of MMP‐9 and a high ratio of MMP‐9 to MMP‐2 in short‐term prostate epithelial cultures is of potential diagnostic and prognostic significance. © 1996 Wiley‐Liss, Inc.