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Homozygous deletions of p16 INK4 occur frequently in bilharziasis‐associated bladder cancer
Author(s) -
Tamimi Yahya,
Bringuier Pierre Paul,
Smit Frank,
van Bokhoven Adrie,
Abbas Ahmed,
Debruyne Frans M.J.,
Schalken Jack A.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19961009)68:2<183::aid-ijc7>3.0.co;2-u
Subject(s) - bladder cancer , biology , cancer , pathology , medicine , cancer research , genetics
We have studied p16 INK4 mutation (by PCR‐SSCP) and deletion (by Southern blotting and/or multiplex PCR) in a series of 47 bilharziasis‐associated tumors from Egypt and compared the results with those obtained on a series of 17 established bladder cell lines and non‐bilharziasis‐associated bladder cancers from the Netherlands. In the cell lines we found 9 homozygous deletions and 1 mutation (59% of p16 INK4 alterations in cell lines), whereas in cases from the Netherlands deletions were found in 4 of 22 samples. No mutations were detected in the 46 samples screened. Interestingly, in bilharziasis‐associated bladder cancer, deletions were present in 23 samples and mutations in a further 2 cases (53% of p16 INK4 alteration in bilharziasis‐associated bladder cancer). No correlation was found between p16 INK4 alteration and histopathological data. Likewise, the same frequency of alteration was found in tumors with different differentiation patterns (squamous, transitional or adenocarcinoma). Three conclusions can be drawn from our findings: ( i ) p16 INK4 alterations are more frequent in cell lines than in primary tumors; ( ii ) in primary bladder tumors (bilharziasis‐associated or not), p16 INK4 deletions are much more frequent than p16 INK4 mutations; ( iii ) p16 INK4 alterations are more frequent in bilharziasis‐associated bladder tumors than in other bladder tumors. This high frequency of deletion is not related to a specific histological type but to the specific etiology of these tumors. © 1996 Wiley‐Liss, Inc.