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Human renal‐cell carcinoma tissue contains dendritic cells
Author(s) -
Thurnher Martin,
Radmayr Christian,
Ramoner Reinhold,
Ebner Susanne,
Böck Günther,
Klocker Helmut,
Romani Nikolaus,
Bartsch Georg
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960927)68:1<1::aid-ijc1>3.0.co;2-v
Subject(s) - dendritic cell , antigen presenting cell , antigen , follicular dendritic cells , immune system , biology , cd40 , cytotoxic t cell , antigen presentation , tumor antigen , immunology , pathology , cancer research , chemistry , t cell , immunotherapy , medicine , in vitro , biochemistry
Immune surveillance of cancer requires antigen‐presenting cells which activate T cells specific for tumor‐associated antigens. We show here that substantial numbers of dendritic cells, which are the most potent antigen‐presenting cells, emigrate from renal‐tumor explants in organ culture. Tumor‐derived dendritic cells presented with all characteristics of mature dendritic cells. Dendritic cells could be identified by typical cytoplasmic projections (=veils). They expressed high levels of MHC products and of the co‐stimulator CD86 (B7‐2). Dendritic cells expressed the CD45RO isoform but not CD45RA. The most important point was that up to 9% of the emigrating leukocytes expressed the CD83 antigen, a specific marker for mature dendritic cells. CD83 + cells were approximately 40‐fold enriched in the tumor tissue as compared to the peripheral blood. In contrast to cultured blood dendritic cells, tumor‐emigrant dendritic cells had a reduced potential to capture soluble antigen, as shown by the exclusion of fluoresceinated Dextran molecules. Finally, in mixed leukocyte reactions, tumor‐derived dendritic cells were able to stimulate naive T cells from cord blood, which is a unique feature of dendritic cells. This study demonstrates that genuine dendritic cells reside in or infiltrate renal‐cell carcinoma tissue. The failure of patients with renal‐cell carcinoma to mount an anti‐tumor immune response despite the presence of professional antigen‐presenting cells in the tumor tissue suggests that tumor‐associated dendritic cells are suppressed in situ , in a similar way to that described for tumor‐infiltrating lymphocytes. © 1996 Wiley‐Liss, Inc.