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Heat shock and cytokines modulate the expression of adhesion molecules on different human gastric‐cancer cell lines
Author(s) -
Hsieh M.C.,
Wu C.W.,
Wu L.H.,
Lui W.Y.,
P'eng F.K.,
Yu C.L.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960904)67:5<690::aid-ijc17>3.0.co;2-9
Subject(s) - cell adhesion molecule , cancer , heat shock protein , cell adhesion , adhesion , cell culture , microbiology and biotechnology , cancer research , cancer cell , biology , cytokine , immunology , cell , chemistry , gene , genetics , organic chemistry
In order to understand the expression and modulation of adhesion molecules (AMs) on the surface of different gastric cancers, we studied 4 gastric‐cancer cell lines including SC‐MI, KATO‐III, AGS and AZ‐521. The expression of E‐cadherin, integrins (β1, β2 and β3), ICAMs (1 and 2), and CD11 (a, b and c) on the cells was detected by flow cytometry. We found that E‐cadherin was only expressed on SC‐MI and KATO‐III. CD29 (β1 integrin) could be found in cells of all 4 lines. CD54 (ICAM‐1) could not be detected in AZ‐521. In contrast, CD18 (β2 integrin), CD61 (β3 integrin), ICAM‐2, CD11a, CD11b and CD11c were all absent from these cells. Heat‐shock treatment (42.5°C, 60 min) enhanced the expression of E‐cadherin, CD29 and CD54 on SC‐M1, and of CD29 on AGS. In addition, TNF‐α (50U/ml) and IL‐1β (10U/ml) modulated the expression of these AMs, like heat‐shock treatment. The increment of these adhesion molecules caused by heat shock, TNF‐α and IL‐1β stimulation on SC‐MI was also confirmed by Western blot analysis. Functionally, these treatments increased the binding between normal human mononuclear cells and SC‐M1 cells. The heat‐shock treatment could induce a significant amount of TNF‐α and IL‐1β release from SC‐MI and KATO‐III, but seemed irrelevant to the expression of AMs. These results suggest that limited adhesion molecules were expressed on the surface of different gastric cancer cells. Heat shock, IL‐1β and TNF‐α may selectively modulate the expression of these 3 molecules on some of the cells, and this is probably related to their anti‐tumor effect. © 1996 Wiley‐Liss, Inc.