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A metastatic neuroblastoma model in SCID mice
Author(s) -
Bogenmann Emil
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960729)67:3<379::aid-ijc12>3.0.co;2-3
Subject(s) - neuroblastoma , medicine , pathology , cancer research , oncology , biology , genetics , cell culture
Neuroblastoma (NB), a neural crest derived tumor in children, shows a characteristic pattern of dissemination that includes adrenal glands, local lymph nodes, bone, liver, skin, and bone marrow. We have reconstructed a similar metastatic pattern in SCID mice following tail vein injection of human NB cells. HTLA230, an NB cell line isolated from a patient with advanced disease, and its NGF receptor ( trkA ) expressing derivative (18–10) cells, consistently disseminated to the liver, the adrenal gland, and the bone marrow, but not the lungs. Metastases in the different organs showed a characteristic hemorrhagic histopathology, and tumors in the bone marrow presented as syncytia‐like cell aggregates, typically seen in patients. Cell lines reestablished from 18–10 derived liver and bone marrow metastases maintained their cellular morphology, growth behavior, N‐ myc overexpression, trkA expression, and functionally responded to NGF treatment, leading to growth arrest and neurite outgrowth. Hence, circulating human NB cells in SCID mice show a similar organ‐specific metastatic potential as seen in patients, independent of trkA expression. © 1996 Wiley‐Liss, Inc.