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Comparison of hypervariable regions (HVR1 and HVR2) in positive‐ and negative‐stranded hepatitis C virus RNA in cancerous and non‐cancerous liver tissue, peripheral blood mononuclear cells and serum from a patient with hepatocellular carcinoma
Author(s) -
Saito Satoru,
Kato Nobuyuki,
Hijikata Makoto,
Gunji Toshiaki,
Itabashi Masayuki,
Kondo Masaaki,
Tanaka Katsuaki,
Shimotohno Kunitada
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960717)67:2<199::aid-ijc9>3.0.co;2-o
Subject(s) - hypervariable region , cirrhosis , hepatocellular carcinoma , hepatitis c virus , virus , peripheral blood mononuclear cell , biology , virology , rna , pathology , antibody , medicine , immunology , cancer research , gene , genetics , in vitro
Hepatitis C virus (HCV) infection is associated with a wide spectrum of liver diseases including cirrhosis and hepatocellular carcinoma (HCC). Although the biological relation between the virus and cirrhosis or HCC is unclear, such variable pathogenicity may be related to the genetic heterogeneity of HCV. Genetic variability of HCV was assessed by determining the nucleotide sequence corresponding to the hypervariable regions (HVR1 and HVR2) of the putative envelope protein (E2/NSI) in positive‐ and negative‐stranded HCV RNA from the cancerous and surrounding non‐cancerous liver tissue, peripheral blood mononuclear cells and serum of a patient with HCC. Nineteen distinct HVRI amino acid sequences (deduced from the nucleotide sequences) were obtained from the patient and could be classified into 5 groups on the basis of the site and time of detection. Some viral isolates with the same HVRI sequence were shown to replicate in both cancerous and non‐cancerous liver tissue, whereas others replicated in HCC tissue only. © 1996 Wiley‐Liss, Inc.