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Expression of Epstein‐Barr virus latent infection genes and oncogenes in lymphoma cell lines derived from pyothorax‐associated lymphoma
Author(s) -
Kanno Hiroyuki,
Yasunaga Yutaka,
Ohsawa Masahiko,
Taniwaki Masafumi,
It chi Keiji,
Naka Norifumi,
Torikai Kei,
Shimoyama Masanori,
Aozasa Katsuyuki
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960703)67:1<86::aid-ijc15>3.0.co;2-a
Subject(s) - biology , microbiology and biotechnology , virus , lymphoma , virology , epstein–barr virus , cell culture , gene expression , gene , immunology , biochemistry , genetics
Malignant lymphomas frequently develop in the pleural cavity of patients with long‐standing pyothorax. The term pyothorax‐associated lymphoma (PAL) has been proposed for this type of tumor. Most PALs are diffuse lymphomas of the B‐cell type and contain Epstein‐Barr virus (EBV) DNA. We have established 2 lymphoma cell lines from biopsy specimens of PAL cases, OPL‐1 and OPL‐2, and examined their growth characteristics and the expression of EBV latent infection genes and oncogenes. OPL‐2 exhibited a more rapid growth and higher saturation density than OPL‐1, and only OPL‐2 exhibited colony‐forming activity in soft agar. OPL‐1 and ‐2 were positive for B‐cell differentiation markers and showed clonal surface immunoglobulins. Both lines contained a single predominant form of episomal EBV DNA, indicating clonal cellular proliferation of an EBV‐infected progenitor cell. OPL‐1 and ‐2 contained type B and A EBV genome, respectively. Expression of EBV nuclear antigen (EBNA)2 mRNA and protein was detected by Northern and Western blot analysis in OPL‐1, but not in OPL‐2. On the other hand, the expression of latent membrane protein (LMP) I mRNA in both OPL‐1 and ‐2 was extremely weak and detectable only by reverse transcription‐polymerase chain reaction. Protein expression of LMPI was not observed by Western blot analysis or immunocytochemistry. Both lines expressed c‐myc mRNA. Only OPL‐1 expressed mRNA of c‐fgr , an oncogene whose expression is upregulated by EBNA2. Both OPLs expressed bcl ‐2 mRNA without detectable expression of LMPI protein. © 1996 Wiley‐Liss, Inc.