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Tetraphenylphosphonium chloride induced mr‐visible lipid accumulation in a malignant human breast cell line
Author(s) -
Delikatny Edward J.,
Roman Sandrine K.,
Hancock Rebecca,
Jeitner Thomas M.,
Lander Catherine M.,
Rideout Darryl C.,
Mountford Carolyn E.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960703)67:1<72::aid-ijc13>3.0.co;2-e
Subject(s) - phosphocholine , lipid droplet , chemistry , cell culture , lysine , catabolism , cationic polymerization , lipid metabolism , cell , phospholipid , metabolism , biochemistry , biology , amino acid , phosphatidylcholine , organic chemistry , membrane , genetics
The effect of the cationic lipophilic phosphonium salt tetraphenylphosphonium chloride (TPP) on a human malignant breast cell line, DU4475, was monitored with proton nuclear magnetic resonance ( 1 H MRS). TPP caused a dose‐ and time‐dependent increase in resonances arising from MR‐visible lipid as measured by the CH 2 /CH 3 ratio in the I‐dimensional 1 H MR spectrum. Two‐dimensional MRS identified increases in the glycerophosphocholine/lysine cross‐peak ratio and corresponding decreases in the phosphocholine/lysine ratio in a dose‐dependent fashion in TPP‐treated cells. Lipid metabolic changes are discussed in the light of other MR experiments, and the data indicate that accumulation of MR‐visible lipids may arise from the rearrangement of phospholipids accompanying mitochondrial destruction or from the catabolism of phospholipids associated with early events in the cytotoxic process. © 1996 Wiley‐Liss, Inc.