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p53 accumulation and mutation are prognostic indicators of poor survival in human gastric carcinoma
Author(s) -
Lim Berenice H. G.,
Soong Richie,
Grieu Fabienne,
Robbins Peter D.,
House Anthony K.,
Iacopetta Barry J.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960621)69:3<200::aid-ijc9>3.0.co;2-3
Subject(s) - immunostaining , pathology , biology , mutation , gene mutation , univariate analysis , carcinoma , survival analysis , single strand conformation polymorphism , cancer research , immunohistochemistry , population , lymph node , gene , medicine , multivariate analysis , genetics , environmental health
The aim of our study was to examine the prognostic significance of p53 protein accumulation and gene mutation in a series of 116 gastric carcinomas from a low incidence population. Formalin‐fixed, paraffin‐embedded tumour sections were used to investigate p53 protein accumulation by immunostaining with monoclonal antibody (MAb) DO‐7 and p53 gene mutation by single‐strand conformation polymorphism analysis of exons 5‐8. Nuclear p53 accumulation was detected in 23% of tumours and mutation in 28%. Concordance between the 2 alterations was observed in 73% of cases. p53 protein accumulation was more frequent in tumours with lymph node metastasis, while p53 mutations were more frequent in tumours from older patients. The histopathological parameters of depth of invasion, grade and histological type showed no significant associations with either p53 alteration. In univariate analysis, both alterations were associated with significantly shortened patient survival. The 5‐year survival rate for patients with a p53 mutation was 9% compared to 42% for those without a mutation. In multivariate analysis adjusted for the other histopathological parameters, p53 gene mutation but not immunohistochemically‐detected p53 protein accumulation was an independent prognostic indicator of poor survival in gastric carcinoma. © 1996 Wiley‐Liss, Inc.