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Novel HPV types present in oral papillomatous lesions from patients with HIV infection
Author(s) -
Völter Christiane,
He Yukai,
Delius Hajo,
RoyBurman Arup,
Greenspan John S.,
Greenspan Deborah,
de Villiers EthelMichele
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960516)66:4<453::aid-ijc7>3.0.co;2-v
Subject(s) - virology , polymerase chain reaction , hpv infection , southern blot , virus , population , atypia , medicine , viral disease , biology , pathology , dna , cancer , gene , cervical cancer , genetics , environmental health
Patients infected with the human immunodeficiency virus (HIV) often develop multiple papillomatous lesions of the oral cavity. In the present study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern‐blot hybridization in combination with a polymerase chain reaction designed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high‐risk population, were subsequently divided into 57 biopsies originating from patients with confirmed HIV infection and 10 biopsies from patients with unknown HIV status. Each sample was amplified with 7 different combinations of degenerate primers. All amplified products were sequenced. HPV DNA sequences were detected in 67% (45/67) of the samples. HPV 7 (19%) and HPV 32 (28%) were the predominant HPV types. HPV 32 was present in 2/4 fibromas tested. Two new HPV types, HPV 72 and HPV 73, were identified in oral warts with atypia. The complete genomes of these viruses were cloned and sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69. © 1996 Wiley‐Liss, Inc.