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Hyperthermia induces apoptosis in malignant fibrous histiocytoma cells in vitro
Author(s) -
Yonezawa Masato,
Otsuka Takanobu,
Matsui Nobuo,
Tsuji Hideki,
Kato Kohichi H.,
Moriyama Akihiko,
Kato Taiji
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960503)66:3<347::aid-ijc14>3.0.co;2-8
Subject(s) - in vitro , apoptosis , hyperthermia , medicine , cancer research , pathology , chemistry , biochemistry
The effect of mild hyperthermia on a cultured rat malignant fibrous histiocytoma (MFH) cell line, MFH‐2NR, was investigated. MFH cells in log‐phase (growing phase) were heated at 41°–44°C for 1 hr. Hyperthermic treatment at 41°C did not substantially affect cell proliferation and treatment at 44°C caused necrosis. After hyperthermic treatment at 42° or 43°C, proliferation of MFH cells was arrested and morphological changes characteristic of apoptosis, cell shrinkage accompanying apoptotic bodies and chromatin condensation, became apparent. Hyperthermia‐induced apoptosis was further confirmed by terminal deoxynucleotidyl transferase staining and a ladder pattern on agarose gel electrophoresis. Flow cytometric analysis indicated that the population in the G 1 phase of the cell cycle significantly decreased with a concomitant increase in apoptotic cells, indicating that apoptosis might occur mainly in the G 1 phase population. © 1996 Wiley‐Liss, Inc.