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β 1 ‐integrins dominate cell traffic of leukemic cells in human bone‐marrow stroma
Author(s) -
Van der VeldeZimmermann Detlef,
Smits Veronique A. J.,
Verdaasdonk Marina A. M.,
Rademakers Louk H. P. M.,
Werner Naomi,
Spierings Diana C. J.,
De Weger Roel A.,
Van den Tweel Jan G.,
Joling Piet
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960410)66:2<225::aid-ijc15>3.0.co;2-b
Subject(s) - stromal cell , bone marrow , k562 cells , fibronectin , cell–cell interaction , cell culture , leukemia , haematopoiesis , biology , integrin , cell adhesion molecule , microbiology and biotechnology , cell adhesion , cell , cancer research , stroma , chemistry , immunology , stem cell , extracellular matrix , biochemistry , immunohistochemistry , genetics
Migration patterns of leukemic cells in bone marrow are largely regulated by cell contacts between leukemic cells and stromal cells or extra‐cellular matrix. The mechanism of this interaction with bone‐marrow stromal cells was studied in a human in vitro model. Migration behavior of erythroleukemia cell line K562, derived from a patient with chronic myeloid leukemia, was compared with that of the erythroleukemia cell line HEL92.1.7 and the promyelocytic leukemia cell line HL60 from acute leukemias. Interaction varied between low binding affinity (K562) to intensive cell interaction (HEL92.1.7) followed by invasion into the stromal cell monolayer. Some of the HL60 cells adhered to stromal cells, while the remainder migrated into the stromal cell monolayer. The role of adhesion molecules in these cell interactions was determined. Distinct expression of β 1 ‐integrins ICAM‐I, CD44 and VCAM‐I was detected on the different cell lines. Inhibition studies pointed to a dominant role of VLA‐4‐ and VLA‐5‐mediated interactions. K562 lacked VLA‐4 and a low affinity of the VLA‐5 on these cells resulted in an absence of binding to the bone‐marrow stroma. These results indicate the VLA‐5/fibronectin, VLA‐4/fibronectin and the VLA‐4/VCAM‐I interaction pathways between leukemic cells and bone‐marrow stroma. © 1996 Wiley‐Liss, Inc.