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Restriction of the T‐cell repertoire in tumor‐infiltrating lymphocytes from nine patients with renal‐cell carcinoma relevance of the CDR3 length analysis for the identification of in situ clonal T‐cell expansions
Author(s) -
Puisieux Isabelle,
Bain Christine,
Merrouche Yacine,
Malacher Philippe,
Kourilsky Philippe,
Even Jos,
Favrot Marie
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960410)66:2<201::aid-ijc11>3.0.co;2-f
Subject(s) - renal cell carcinoma , t cell receptor , biology , t cell , immunotherapy , antigen , tumor infiltrating lymphocytes , immune system , pathology , metastasis , immunology , cancer research , cancer , medicine , genetics
Renal‐cell carcinoma (RCC) is one of the human cancers which respond best to immunotherapy. To better characterize the mechanism of the immune response in RCC, we analyzed the T‐cell receptor (TCR) β‐chain repertoire in primary RCC, metastases and paired peripheral blood lymphocytes (PBL) from 9 patients. For 3 of these, we also analyzed T cells recovered from normal kidney, or from tumor‐involved lymph nodes as well as tumor‐infiltrating lymphocytes (TIL) expanded in vitro for adoptive immunotherapy. The initial semi‐quantitative RT‐PCR method for definition of the Vβ gene usage was not informative enough to distinguish intratumoral clonal T‐cell expansions. In contrast, the length pattern analysis of the complementary determining regions 3 (CDR3) allowed oligoclonal T‐cell populations to be detected in fresh TIL from the 9 patients with RCC. Furthermore, these oligoclonal TIL populations were not present in normal renal tissue, autologous PBL or tumor‐involved lymph nodes. Different clonal T‐cell expansions were identified in the primary tumor and in a pulmonary metastasis from the same patient. The detection of clonal T‐cell populations observed in RCC suggests an in situ expansion in response to potential tumor antigens. This report provides an overall and accurate description of the T‐cell repertoire in a significant number of samples from patients with RCC. © 1996 Wiley‐Liss, Inc.