Premium
Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co‐culture of human osteosarcoma cells and human fibroblasts: Gelatinase‐B production was stimulated via up‐regulation of fibroblast growth factor (FGF) receptor
Author(s) -
Kurogi Takao,
Nabeshima Kazuki,
Kataoka Hiroaki,
Okada Yasunori,
Koono Masashi
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960328)66:1<82::aid-ijc15>3.0.co;2-d
Subject(s) - gelatinase , basic fibroblast growth factor , gelatinase a , fibroblast activation protein, alpha , fibroblast , cell culture , collagenase , biology , stimulation , microbiology and biotechnology , growth factor , matrix metalloproteinase , endocrinology , receptor , biochemistry , enzyme , cancer , genetics
Co‐cultures of human osteosarcoma Takase (OST) cells with various human fibroblasts derived from surgical specimens stimulated production of gelatinase B (92‐kDa type‐IV collagenase, MMP‐9), tissue inhibitors of metalloproteinase (TIMP)‐1, and TIMP‐2 when compared to cultures of individual cells. The maximum stimulation of gelatinase‐B production occurred at a cellular ratio of I:I. Conditioned media from several fibroblast cultures stimulated OST cells to produce gelatinase B, TIMP‐1 and TIMP‐2, but not vice versa. Among various recombinant growth factors or cytokines, tumor necrosis factor (TNF)‐α stimulated gelatinase‐B production in cultures of OST cells alone, while recombinant basic fibroblast growth factor (bFGF) stimulated gelatinase‐B production in co‐cultures of OST cells with skin fibroblasts but not in individual cultures of each cell type. In the co‐cultures, gelatinase‐B production was inhibited by anti‐bFGF monoclonal antibody (MAb), but not by anti‐TNF‐αMAb. This co‐culture‐specific stimulation of gelatinase‐B production by bFGF was associated with increased expression of the FGF receptor in the co‐culture. © 1996 Wiley‐Liss, Inc.