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Tumor necrosis factor‐A expression in human primary malignant melanoma and its relationship to tumor infiltration by CD3 + cells
Author(s) -
Sander Birgitta,
Boeryd Bernt
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960328)66:1<42::aid-ijc8>3.0.co;2-z
Subject(s) - tumor necrosis factor alpha , melanoma , cd3 , infiltration (hvac) , cytokine , immunohistochemistry , immune system , pathology , stain , necrosis , cancer research , tumor infiltrating lymphocytes , medicine , biology , staining , immunology , immunotherapy , cd8 , materials science , composite material
Optimal conditions for immunohistochemical staining of tumor necrosis factor‐A (TNF‐A) in paraffin‐embedded tissue sections were established to investigate TNF‐A expression in human primary malignant melanomas. Seventeen malignant melanomas of the nodular (NMM) and superficially spreading (SSM) subtypes were analyzed. Twelve of these were TNF‐A + , while 5 did not stain for the cytokine. To evaluate how TNF‐A expression affected the immune response to the tumors, infiltration by CD3 + and mac387 + cells was investigated in NMM. TNF‐A expression seemed to selectively affect the capability of T cells to infiltrate the tumors since TNF‐A + tumors were found to have significantly lower levels of infiltrating CD3 + cells, while there was no difference in numbers of mac387 + cells. These results demonstrate that TNF‐A is variably expressed in primary malignant melanoma in vivo and that the T‐cell response to TNF‐A‐expressing NMM is inhibited. © 1996 Wiley‐Liss, Inc.