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Detection of human T‐lymphotrophic virus type‐I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT‐PCR/ISH)
Author(s) -
Ohshima Koichi,
Suzumiya Junji,
Izumo Shuji,
Mukai Yasuo,
Tashiro Kohtaro,
Kikuchi Masahiro
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960328)66:1<18::aid-ijc4>3.0.co;2-1
Subject(s) - in situ hybridization , polymerase chain reaction , reverse transcriptase , microbiology and biotechnology , biology , reverse transcription polymerase chain reaction , lymph , virus , dna , virology , dna polymerase , messenger rna , pathology , gene , medicine , genetics
To examine the relationship between human T‐lymphotrophic virus type I (HTLV‐I) proviral DNA and its expression in the lymph nodes, HTLV‐I DNA and tax/rex mRNA were directly amplified by polymerase chain reaction in situ hybridization (PCR/ISH), and reverse transcription (RT)‐PCR/ISH [RT‐PCR/ISH]. We studied 24 lymph nodes from patients with adult T‐cell leukemia/lymphoma (ATLL), incipient ATLL (I‐ATLL), and HTLV‐I‐associated lymphadenitis dermatopathic type (HAL‐D) and enlarged paracortical type (HAL‐EP). In ATLL, 40–60% of the nucleated cells were positive for HTLV‐I proviral DNA by PCR/ISH, while in I‐ATLL and HAL, respectively 5–20% and less than 1–5% of cells were positive. The number of mRNA‐expressing cells was smaller than that of the proviral DNA‐positive cells. The mRNA‐expressing cells varied in number among the ATLL and I‐ATLL cases, while they were only rarely observed in HAL‐D and HAL‐EP. These results show that HTLV‐I infection and activation might increase with malignant transformation of the target T helper cells. © 1996 Wiley‐Liss, Inc.