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Colonization of human lung grafts in SCID‐hu mice by human colon carcinoma cells
Author(s) -
SampsonJohannes Adam,
Wang Wei,
Shtivelman Emma
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960315)65:6<864::aid-ijc26>3.0.co;2-2
Subject(s) - biology , cell culture , in vivo , pathology , lewis lung carcinoma , cancer research , immunohistochemistry , cell , phenotype , carcinoma , lung , cell adhesion molecule , cell adhesion , metastasis , cancer , immunology , medicine , gene , genetics , biochemistry , microbiology and biotechnology
Human colon carcinoma cell lines were examined in a colonization assay using SCID‐hu mice engrafted with human fetal lung (HFL) tissues. Cell lines SW620 and COLO 320DM, derived from metastatic tumors, colonized HFL grafts after i.v. injection into SCID‐hu mice. Cell lines SW480 and T34 initiated from primary colon tumors were unable to colonize HFL grafts. The ability to colonize HFL grafts but not mouse lungs of SCID‐hu‐L mice correctly reflects the clinical origin of these human colon carcinoma cell lines. Expression of a number of cell adhesion molecules was examined on SW480, SW620 and in vivo selected highly aggressive variants of SW620. NCAM and integrin α3 expressed on the surface of SW480 cells were lost from metastatic cells, while carbohydrate ligands sialyl Lewis x and a, previously shown to be upregulated in metastatic colorectal tumors, were expressed at higher levels on colonizing cells. Unlike SW480, SW620 and its in vivo selected variants expressed RNA for calcium binding protein calbindin‐D28K, a neuroendocrine marker. Acquisition of neuroendocrine features might be of potential importance in the development of the metastatic phenotype. © 1996 Wiley‐Liss, Inc.